Introduction to CRFT

///Introduction to CRFT
Introduction to CRFT2017-11-07T18:06:18+00:00


Cell Rich Fat Transfer is a simple, fast, gentle and low cost fat harvesting and transfer method, which yields quality re-injectable adipose with high potency of adipose derived regenerative cells (ADRC’s).

CRFTThe Cell Rich Fat Transfer (CRFT) protocol suggests the use of a special harvesting cannula called the Super-G™, gently removes small adipose cell clusters, which have been loosened inside the body via mechanical dissociation of power assisted liposuction devices like MicroAire PAL, Euromi Lipomatic, and Ultrasound.

Cell Rich Fat

A recent study showed that mechanical dissociation yields a “superior” volume of adipose derived regenerative cells (ADRC’s) compared to standard liposuction. ¹ This yield is due to the unique cannula we use for power assisted liposuction devices.  Additional analysis comparing mechanical dissociation to standard liposuction showed mechanical lipoaspirate to yield twice the volume of ADRC’s and three times the volume of mesenchymal cells. ²  Further, the harvested ADRC’s have been shown to be highly viable.¹˒²  The consistent volume of highly viable ADRC’s present in mechanically harvested fat makes the CRFT method of autologous fat transfer superior to other methods, including those including enzymatic processing.

Stem Cell Content Influences Outcomes

Studies have shown that a high population of ADRC’s present within transplanted fat cells will increase survival rates and volume retention, due to enhanced angiogenesis and adipose cell replacement.³ The high yield of ADRC’s resulting from the in vivo dissociation of regenerative cells makes fat harvested using the CRFT protocol an ideal re-injectable material for achieving predictable outcomes.

Decanting vs. Centrifugation

Recent studies have shown that decanting is preferable to centrifugation for fat survivability.⁴   Centrifugation tends to damage fragile cells and has also been shown to deplete the separated fat of beneficial ADRC’s.⁴  Therefore, the CRFT protocol suggests that sterile decantation will preserve the integrity of fat cells and prevent the separation of free floating ADRC’s.


1. Baptista, et al., An alternative method for the isolation of mesenchymal stromal cells derived from lipoaspirate samples, Cytotherapy (2009) Vol. II, No. 6, 706-‐715.
2. Angeloni, Nutational Infrasonic Liposculpture (NIL) Adipose Derive Regenerative Cell Concentration and Viability Study Summary, non-‐published November 2011.
3. Yoshimura, et. al, Cell-‐assisted lipotransfer for cosmetic breast augmentation: Supportive use of adipose-‐derived stem/stromal cells. Aesthetic Plastic Surgery (2008) 32: 48-‐55
4. Conde-‐Green, Effects of centrifugation on cell composition and viability of aspirated adipose tissue processed for transplantation. Aesthetic Surgery Journal, March 2010, Vol. 30, No. 2, 249-‐255